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GE Healthcare
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Accurate Chemical & Scientific Corporation
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Sinsheimer Laboratories
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Spinco Biotech
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Spafas Inc
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Bio-Rad
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Spinco Biotech
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Thermo Fisher
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Santa Cruz Biotechnology
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Beckman Coulter
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Beckman Coulter
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Image Search Results
Journal: PLoS Pathogens
Article Title: Binding of Glutathione to Enterovirus Capsids Is Essential for Virion Morphogenesis
doi: 10.1371/journal.ppat.1004039
Figure Lengend Snippet: Effect of TP219-, BSO- and mock treatment on the levels of 5S protomers and 14S pentamers as determined by immunoprecipitation ( A–B ) and immunoblotting analysis ( C–E ). ( A–B ) Two consecutive fractions of a 6 to 25% gradient were pooled, immunoprecipitated using polyclonal anti-enterovirus antibodies and assayed for radioactivity. Different immunoprecipitated fractions (bars) are overlaid on TCA-precipitated fractions (lines) (derived from ) and the amount of radioactivity is expressed as % of total signal to normalize for the incorporation efficiency. Immunoprecipitated fractions of untreated cultures ( A and B ) are indicated in white; TP219- ( A ) or BSO- ( B ) treated cultures in black. Cartoon representations of the 5S and 14S assembly intermediates are indicated as well. ( C–E ) Two consecutive fractions of a 6 to 25% gradient were pooled, TCA-precipitated and subjected to Western blotting analysis using a monoclonal anti-VP1 antibody. Both the immunoblot (below) and quantification of the immunoblot (top) of mock ( C ), TP219- ( D ) and BSO- ( E ) treated cultures are depicted. Sucrose density is indicated as well and expressed as g/cm 3 .
Article Snippet: For immunoprecipitation of sucrose gradients we used
Techniques: Immunoprecipitation, Western Blot, Radioactivity, Derivative Assay